Wasp的尾巴與Cell Migration有關(guān)
How Cdc42 and acidic
phospholipids might activate WASP. Inset shows an actin-filament
halo (red) surrounding a vesicle (green). Photo courtesy of Henry Higgs and Tom Pollard, Salk Institute, La Jolla,California, USA.
細(xì)胞的移動(dòng)需要肌動(dòng)蛋白的多聚化,外界的刺激究竟是如何促使細(xì)胞發(fā)生改變的呢?有證據(jù)表明WASP(Wiskott–Aldrich
syndrome protein)家族蛋白可能是信號(hào)傳遞者,但對(duì)于科學(xué)家來說,細(xì)胞移動(dòng)的詳細(xì)機(jī)制始終難以捉摸。The
Journal of Cell Biology上的兩篇文章指出脂類與蛋白質(zhì)相互聯(lián)合并激活了WASP。
肌動(dòng)蛋白的成核現(xiàn)象有Arp2/3復(fù)合物激活,Arp2/3是由WASP活化的。一種小GTPase
Cdc42及一種磷脂PtdIns(4,5)P2活化了WASP。
Henry
Higgs and Tom Pollard從小牛胸腺中純化了haematopoietic-cell特異性的WASP,他們發(fā)現(xiàn)純化的WASP是無活性的,但當(dāng)包含了PtdIns(4,5)P2的微團(tuán),cdc42或包含另一種磷脂,磷脂酰絲氨酸的小泡存在時(shí),WASP能將肌動(dòng)蛋白多聚化了。此過程需要的cdc42是結(jié)合了GTP,被活化的。
Rohatgi and colleagues研究了另一種廣泛表達(dá)的N-WASP的重組蛋白,發(fā)現(xiàn)cdc42而不是PtdIns(4,5)P2能夠部分激活N-WASP,而兩者聯(lián)合能完全激活N-WASP。他們對(duì)WASP的C末端進(jìn)行了分析,發(fā)現(xiàn)了如圖的精巧機(jī)制,解釋了WASP的自抑制機(jī)制,也解釋了cdc42和磷脂對(duì)WASP的激活機(jī)制。
相關(guān)文章:
ORIGINAL RESEARCH PAPER
Higgs , H. N. & Pollard,T. D. Activation by Cdc42 and PIP2 of Wiskott–Aldrich syndrome
protein (WASP) stimulates actin nucleation by Arp2/3 complex. J. Cell
Biol. 150, 1311–1320 (2000). [ PubMed] PubMed
Rohatgi, R. et al. Mechanism
of N-WASP activation by CDC42 and Phosphatidylinositol
4,5-bisphosphate. J. Cell Biol. 150, 1299 –1309 (2000). [ PubMed]
PubMed
FURTHER READING
Cameron, L. A. et al. Secrets
of actin-based motility revealed by a bacterial pathogen. Nature Rev.
Mol. Cell Biol. 1, 110–119 (2000) [At a glance]
Kim, A. S., Kakalis, L.T.,Abdul-Manan, N., Liu, G.A. & Rosen, M.K. Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein. Nature
404, 151- 158 (2000). [PubMed] PubMed
Blanchoin, L. et al. Direct
observation of dendritic actin filament networks nucleated by Arp2/3
complex and WASP/Scar proteins. Nature 404 , 1007-1011 (2000). [PubMed]
PubMed, http://www.www.srpcoatings.com
phospholipids might activate WASP. Inset shows an actin-filament
halo (red) surrounding a vesicle (green). Photo courtesy of Henry Higgs and Tom Pollard, Salk Institute, La Jolla,California, USA.
細(xì)胞的移動(dòng)需要肌動(dòng)蛋白的多聚化,外界的刺激究竟是如何促使細(xì)胞發(fā)生改變的呢?有證據(jù)表明WASP(Wiskott–Aldrich
syndrome protein)家族蛋白可能是信號(hào)傳遞者,但對(duì)于科學(xué)家來說,細(xì)胞移動(dòng)的詳細(xì)機(jī)制始終難以捉摸。The
Journal of Cell Biology上的兩篇文章指出脂類與蛋白質(zhì)相互聯(lián)合并激活了WASP。
肌動(dòng)蛋白的成核現(xiàn)象有Arp2/3復(fù)合物激活,Arp2/3是由WASP活化的。一種小GTPase
Cdc42及一種磷脂PtdIns(4,5)P2活化了WASP。
Henry
Higgs and Tom Pollard從小牛胸腺中純化了haematopoietic-cell特異性的WASP,他們發(fā)現(xiàn)純化的WASP是無活性的,但當(dāng)包含了PtdIns(4,5)P2的微團(tuán),cdc42或包含另一種磷脂,磷脂酰絲氨酸的小泡存在時(shí),WASP能將肌動(dòng)蛋白多聚化了。此過程需要的cdc42是結(jié)合了GTP,被活化的。
Rohatgi and colleagues研究了另一種廣泛表達(dá)的N-WASP的重組蛋白,發(fā)現(xiàn)cdc42而不是PtdIns(4,5)P2能夠部分激活N-WASP,而兩者聯(lián)合能完全激活N-WASP。他們對(duì)WASP的C末端進(jìn)行了分析,發(fā)現(xiàn)了如圖的精巧機(jī)制,解釋了WASP的自抑制機(jī)制,也解釋了cdc42和磷脂對(duì)WASP的激活機(jī)制。
相關(guān)文章:
ORIGINAL RESEARCH PAPER
Higgs , H. N. & Pollard,T. D. Activation by Cdc42 and PIP2 of Wiskott–Aldrich syndrome
protein (WASP) stimulates actin nucleation by Arp2/3 complex. J. Cell
Biol. 150, 1311–1320 (2000). [ PubMed] PubMed
Rohatgi, R. et al. Mechanism
of N-WASP activation by CDC42 and Phosphatidylinositol
4,5-bisphosphate. J. Cell Biol. 150, 1299 –1309 (2000). [ PubMed]
PubMed
FURTHER READING
Cameron, L. A. et al. Secrets
of actin-based motility revealed by a bacterial pathogen. Nature Rev.
Mol. Cell Biol. 1, 110–119 (2000) [At a glance]
Kim, A. S., Kakalis, L.T.,Abdul-Manan, N., Liu, G.A. & Rosen, M.K. Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein. Nature
404, 151- 158 (2000). [PubMed] PubMed
Blanchoin, L. et al. Direct
observation of dendritic actin filament networks nucleated by Arp2/3
complex and WASP/Scar proteins. Nature 404 , 1007-1011 (2000). [PubMed]
PubMed, http://www.www.srpcoatings.com
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