王杰軍.pdf
http://www.www.srpcoatings.com
| 第1頁 |
| 第3頁 |
參見附件(291kb)。
中國臨床腫瘤學(xué)教育專輯 (2007) 341
大規(guī)模胃癌組織芯片的構(gòu)建以及胃癌預(yù)后因素的分析
上海市長征醫(yī)院腫瘤科 王杰軍 于觀貞 陳 穎 王 喜 潘 軍
摘要 目的:探尋組織芯片在胃癌研究中的意義,并初步研究影響預(yù)后的臨床病理因素。方
法:收集 1072 例胃癌手術(shù)切除標(biāo)本,利用組織芯片儀構(gòu)建組織芯片,結(jié)合免疫組化,檢測 P53
和 PCNA 的表達(dá)。并用 Kaplan-Meier 分析和多因素 Cox 回歸等統(tǒng)計(jì)方法分析影響胃癌患者預(yù)后
的相關(guān)因素。結(jié)果:成功構(gòu)建了含有 1072 例胃癌信息的組織芯片,P53 和 PCNA 在胃癌中的表
達(dá)率顯著高于正常組織中的表達(dá),分別為55.1%和 89.3%,進(jìn)展期胃癌中的表達(dá)率顯著高于早期
胃癌中的表達(dá)。P53的過度表達(dá)與年齡、男性、貧血、腺癌、分化、漿膜侵犯和淋巴結(jié)轉(zhuǎn)移高度
相關(guān);而 PCNA則與年齡、腫瘤部位、腺癌、分化、漿膜侵犯、淋巴結(jié)轉(zhuǎn)移以及TNM高度相關(guān)。
單因素分析當(dāng)中,年齡、貧血、腫瘤大小、分化、腫瘤分期、侵犯深度、淋巴結(jié)轉(zhuǎn)移、手術(shù)方式、切緣、術(shù)中輸血以及 PCNA 均與患者惡性預(yù)后相關(guān)。而在 Cox 回歸多因素分析當(dāng)中,年齡、分
化、腫瘤分期、淋巴結(jié)轉(zhuǎn)移、手術(shù)方式、術(shù)中輸血以及PCNA與患者惡性預(yù)后相關(guān)。結(jié)論:P53
和 PCNA 在胃癌的發(fā)生和發(fā)展的過程中起到重要作用;患者、腫瘤、手術(shù)情況和生物學(xué)因素在
內(nèi)的綜合因素影響了胃癌患者的預(yù)后。
The Construction of Tissue Microarrays of Gastric Carcinoma And Analysis of
Clinicopathological Prognostic Factors of Chinese Gastric Cancer Patietns
WANG Jie-Jun*, YU Guan-Zhen, CHEN Ying,. 1.Department of Oncology, Chang-zheng Hospital,Shanghai 200070, China; 2. Department of Pathology, Chang-hai Hospital, Shanghai 200433, China
Abstract Objective: To construct tissue microarrays of human gastric carcinoma containing large
proportion of cases and to investigate prognostic factors of gastric carcinoma. METHODS: Clinical
data of 1072 patients with gastric carcinoma, received radical operation from Jan. 2001 to May. 2005
were used to construct the tissue microarrays and analyzed retrospectively. Life table method was used
to analyze survival rate, Kaplan-Meier was used for univariate analysis, and Cox regression model was
used for multivariate analysis. Immunohistochemistry was used to detect the expression of p53 and
PCNA. RESULTS: Tissue microarrays containing 1072 cases were successfully constructed. P53 and
PCNA was overexpressed in the primary tumor, 55.1% and 89.3%, respectively. Overexpression of P53
was significantly associated with age, male, anemia, tumor type, differentiation, gastric wall invasion,nodal metastasis and TNM stage. PCNA overexpression had a significant relationship with age, tumor
type, tumor location, differentiation, gastric wall invasion, nodal metastasis and TNM stage. Univariate
analysis showed that age, anemia, tumor size, tumor stage, invasion, nodal metastasis, surgical methods,tumor margin, blood transfusion and PCNA were prognostic factors of gastric carcinoma. Multivariate
analysis showed that age, differentiation, pTNM stage, nodal metastasis, surgical methods, blood
transfusion and PCNA were independent prognostic factors of gastric carcinoma. CONCLUSIONS:
Tissue microarrays provide a useful platform for the scientific research of gastric cancer. P53 and
PCNA play a critical role in the development and progress of gastric carcinoma. Age, differentiation,pTNM stage, nodal metastasis, surgical methods, blood transfusion and PCNA are the most significant
factors influencing prognosis of gastric carcinoma patients after radical operation. 342 中國臨床腫瘤學(xué)教育專輯 (2007)
世界范圍內(nèi)胃癌的發(fā)病率有所下降,國內(nèi)其發(fā)病率已降至第三位,次于肝癌和肺癌。然而其死亡率
一直居高不下,處于第二位,僅次于肺癌。根據(jù)IARCPress2004 年報(bào)道 2002年中國胃癌人數(shù)占到全世界
的 42%以上,因此胃癌嚴(yán)重威脅人們的身體健康并浪費(fèi)社會(huì)資源。研究影響胃癌患者預(yù)后的臨床病理因
素以及探尋行之有效的研究方法,是十分迫切和必要的。
醫(yī)學(xué)分子生物學(xué)技術(shù)的發(fā)展為胃癌的研究提供了許多非常有效的研究工具,其中組織芯片技術(shù)尤為
特別 ......
大規(guī)模胃癌組織芯片的構(gòu)建以及胃癌預(yù)后因素的分析
上海市長征醫(yī)院腫瘤科 王杰軍 于觀貞 陳 穎 王 喜 潘 軍
摘要 目的:探尋組織芯片在胃癌研究中的意義,并初步研究影響預(yù)后的臨床病理因素。方
法:收集 1072 例胃癌手術(shù)切除標(biāo)本,利用組織芯片儀構(gòu)建組織芯片,結(jié)合免疫組化,檢測 P53
和 PCNA 的表達(dá)。并用 Kaplan-Meier 分析和多因素 Cox 回歸等統(tǒng)計(jì)方法分析影響胃癌患者預(yù)后
的相關(guān)因素。結(jié)果:成功構(gòu)建了含有 1072 例胃癌信息的組織芯片,P53 和 PCNA 在胃癌中的表
達(dá)率顯著高于正常組織中的表達(dá),分別為55.1%和 89.3%,進(jìn)展期胃癌中的表達(dá)率顯著高于早期
胃癌中的表達(dá)。P53的過度表達(dá)與年齡、男性、貧血、腺癌、分化、漿膜侵犯和淋巴結(jié)轉(zhuǎn)移高度
相關(guān);而 PCNA則與年齡、腫瘤部位、腺癌、分化、漿膜侵犯、淋巴結(jié)轉(zhuǎn)移以及TNM高度相關(guān)。
單因素分析當(dāng)中,年齡、貧血、腫瘤大小、分化、腫瘤分期、侵犯深度、淋巴結(jié)轉(zhuǎn)移、手術(shù)方式、切緣、術(shù)中輸血以及 PCNA 均與患者惡性預(yù)后相關(guān)。而在 Cox 回歸多因素分析當(dāng)中,年齡、分
化、腫瘤分期、淋巴結(jié)轉(zhuǎn)移、手術(shù)方式、術(shù)中輸血以及PCNA與患者惡性預(yù)后相關(guān)。結(jié)論:P53
和 PCNA 在胃癌的發(fā)生和發(fā)展的過程中起到重要作用;患者、腫瘤、手術(shù)情況和生物學(xué)因素在
內(nèi)的綜合因素影響了胃癌患者的預(yù)后。
The Construction of Tissue Microarrays of Gastric Carcinoma And Analysis of
Clinicopathological Prognostic Factors of Chinese Gastric Cancer Patietns
WANG Jie-Jun*, YU Guan-Zhen, CHEN Ying,. 1.Department of Oncology, Chang-zheng Hospital,Shanghai 200070, China; 2. Department of Pathology, Chang-hai Hospital, Shanghai 200433, China
Abstract Objective: To construct tissue microarrays of human gastric carcinoma containing large
proportion of cases and to investigate prognostic factors of gastric carcinoma. METHODS: Clinical
data of 1072 patients with gastric carcinoma, received radical operation from Jan. 2001 to May. 2005
were used to construct the tissue microarrays and analyzed retrospectively. Life table method was used
to analyze survival rate, Kaplan-Meier was used for univariate analysis, and Cox regression model was
used for multivariate analysis. Immunohistochemistry was used to detect the expression of p53 and
PCNA. RESULTS: Tissue microarrays containing 1072 cases were successfully constructed. P53 and
PCNA was overexpressed in the primary tumor, 55.1% and 89.3%, respectively. Overexpression of P53
was significantly associated with age, male, anemia, tumor type, differentiation, gastric wall invasion,nodal metastasis and TNM stage. PCNA overexpression had a significant relationship with age, tumor
type, tumor location, differentiation, gastric wall invasion, nodal metastasis and TNM stage. Univariate
analysis showed that age, anemia, tumor size, tumor stage, invasion, nodal metastasis, surgical methods,tumor margin, blood transfusion and PCNA were prognostic factors of gastric carcinoma. Multivariate
analysis showed that age, differentiation, pTNM stage, nodal metastasis, surgical methods, blood
transfusion and PCNA were independent prognostic factors of gastric carcinoma. CONCLUSIONS:
Tissue microarrays provide a useful platform for the scientific research of gastric cancer. P53 and
PCNA play a critical role in the development and progress of gastric carcinoma. Age, differentiation,pTNM stage, nodal metastasis, surgical methods, blood transfusion and PCNA are the most significant
factors influencing prognosis of gastric carcinoma patients after radical operation. 342 中國臨床腫瘤學(xué)教育專輯 (2007)
世界范圍內(nèi)胃癌的發(fā)病率有所下降,國內(nèi)其發(fā)病率已降至第三位,次于肝癌和肺癌。然而其死亡率
一直居高不下,處于第二位,僅次于肺癌。根據(jù)IARCPress2004 年報(bào)道 2002年中國胃癌人數(shù)占到全世界
的 42%以上,因此胃癌嚴(yán)重威脅人們的身體健康并浪費(fèi)社會(huì)資源。研究影響胃癌患者預(yù)后的臨床病理因
素以及探尋行之有效的研究方法,是十分迫切和必要的。
醫(yī)學(xué)分子生物學(xué)技術(shù)的發(fā)展為胃癌的研究提供了許多非常有效的研究工具,其中組織芯片技術(shù)尤為
特別 ......
您現(xiàn)在查看是摘要介紹頁,詳見PDF附件(291KB,7頁)。