脈絡(luò)寧注射液對(duì)肢體缺血-再灌注損傷一氧化氮及一氧化氮合酶的影響
王岱君1，王金平1，田華2(濰坊醫(yī)學(xué)院①人體解剖學(xué)教研室，②保健科，山東 濰坊 261042)
【摘要】 目的：探討脈絡(luò)寧注射液對(duì)肢體缺血-再灌注損傷一氧化氮(NO)和一氧化氮合酶(NOS)的影響。方法：健康成年家兔20只，隨機(jī)分為對(duì)照組和實(shí)驗(yàn)組，建立后肢缺血-再灌注損傷動(dòng)物模型。在即將恢復(fù)血流灌注時(shí)，實(shí)驗(yàn)組家兔自耳緣靜脈注射脈絡(luò)寧注射液，對(duì)照組注射等量生理鹽水。在缺血前、缺血后2 h、再灌注后1 h和4 h各自從術(shù)側(cè)股靜脈采血，分離血清，測(cè)定血清NO和NOS的含量；取脛前肌行電鏡觀察。結(jié)果：兩組血清NO含量在缺血后2 h比缺血前均明顯增高(P均<0.05)，再灌注后均明顯降低(P均<0.05)。兩組NOS活性在缺血后2 h比缺血前明顯降低(P均<0.01)，再灌注后比缺血后2 h均明顯增高(P<0.05或P<0.01)，且實(shí)驗(yàn)組明顯高于對(duì)照組(P<0.01和P<0.05)。電鏡觀察可見(jiàn)再灌注后對(duì)照組組織損傷嚴(yán)重，而實(shí)驗(yàn)組較輕。結(jié)論：脈絡(luò)寧注射液能抑制肢體缺血-再灌注損傷NO的過(guò)量產(chǎn)生，提高NOS活性，對(duì)肢體缺血-再灌注損傷具有保護(hù)作用。
【關(guān)鍵詞】 一氧化氮；一氧化氮合酶；脈絡(luò)寧注射液；缺血-再灌注損傷，肢體
Effect of Mailuoning injection (脈絡(luò)寧注射液) on nitric oxide and nitric oxide synthase in rabbits with extremity ischemia/reperfusion injury
WANG Dai-jun1, WANG Jin-ping1, TIAN Hua2.
1. Department of Human Anatomy, Weifang Medical College, Weifang 261042, Shandong, China; 2. Department of Health, Weifang Medical College, Weifang 261042, Shandong, China
【Abstract】 Objective： To investigate the effect of Mailuoning injection(脈絡(luò)寧注射液) on nitric oxide (NO) and nitric oxide synthase (NOS) in rabbits with extremity ischemia/reperfusion injury. Methods： The experimental models of extremity ischemia/reperfusion injury were established in 20 healthy adult rabbits that wererandomly divided into control group and experimental group. Intravenously, normal saline was infused into rabbits of the control group and Mailuoning injection, in experimental group just before blood reperfusion. Before ischemia, 2 hours after ischemia, 1 hour after reperfusion and 4 hours after reperfusion, the samples of blood were taken from the femoral veins respectively for measurements of NO and NOS in serum. Meanwhile the tibialis anterior muscles were harvested for the ultrastructure examinations. Results： The content of NO in serum was obviously increased after ischemia and decreased after reperfusion in both two groups(both P<0.05). NOS activity was decrease significantly 2 hours after ischemia, and significantly increased after reperfusion, especially in the experimental group (P<0.05 or P<0.01). The skeletal muscle injury of the experimental group, after reperfusion, was of a mild degree than that of control group in electron microscope examinations. Conclusion： The Mailuoning injection is able to inhibit excessive production of NO induced by extremity ischemia/reperfusion, and enhance NOS activity, however, it possessesprotective effect on extremity ischemia/reperfusion injury. ......
百拇醫(yī)藥網(wǎng) http://www.www.srpcoatings.com/html/Dir/2005/02/01/52/77/72.htm
您現(xiàn)在查看是摘要頁(yè)，全文長(zhǎng) 11860 字符。