關(guān)鍵詞：粘性放線菌；菌毛；粘附；凝集
摘要 目的 :了解粘放菌兩種菌毛在粘附牙面和與血鏈球菌34凝集中的生物活性。方法 :采用粘放菌Ⅰ型和Ⅱ型菌毛提取物及兩種抗菌毛抗體,通過(guò)它們對(duì)粘放菌粘附唾液包被的羥磷灰石(SHA)的抑制實(shí)驗(yàn)來(lái)判斷兩種菌毛的粘附活性,通過(guò)粘放菌與血鏈球菌34的凝集實(shí)驗(yàn)及兩種抗菌毛抗體對(duì)凝集反應(yīng)的抑制實(shí)驗(yàn)來(lái)確定兩種菌毛的凝集活性。結(jié)果 :①粘放菌Ⅰ型和Ⅱ型菌毛提取物及兩種抗菌毛抗體均能抑制粘放菌對(duì)SHA的粘附;②只有Ⅱ型菌毛能間接引起肉眼可見(jiàn)的血鏈球菌34的凝集反應(yīng),抗Ⅱ型菌毛抗體能抑制凝集反應(yīng),抗Ⅰ型菌毛抗體無(wú)此抑制作用。結(jié)論 :Ⅰ型和Ⅱ型菌毛均有粘附性能;Ⅱ型菌毛有凝集性能,Ⅰ型菌毛無(wú)凝集性能;本課題所采用的菌毛分離法未破壞菌毛的生物活性。
A Study on BiologicalProperties of Fimbriae of A.viscosus
Ⅲ. Adherence Activityof Fimbriae of A.viscosus
Liu Tianjia, Li Wen, Yue Songling
College of Stomatology, West China University of Medical Sciences
Abstract Objective : Toinvestigate the adherence activity of two types of fimbriae of A.viscosus on tooth surfaceor with S.sanguis 34. Methods : The inhibited adherence tests,coaggregation tests and the inhibited coaggregation tests were done. Results : ①The purified type Ⅰ and type Ⅱ fimbriae inhibited the adherence of A.viscosus to salivary-treated hydroxyapatite (SHA) and the two specific IgG to type Ⅰ and type Ⅱ fimbriae blocked the adsorption of strainT14V, strain 5519 and strain 5951 to SHA; ②Only type Ⅱ fimbria indirectly mediated the visible agglutintion of S. sanguis 34 and only IgG to type Ⅱ fimbriae inhibited coaggregation of strain T14V and strain 5915with S. sanguis 34. Conclusion : Type Ⅰ and type Ⅱ fimbriae have adherence ability, and only type Ⅱ fimbria has the agglutinationactivity. Additionally, the methods which were used to prepare fimbriae don't damage thebiological activity of fimbriae.
Key words : A.viscosus S.sanguis fimbriae adhere coaggregation
菌斑是導(dǎo)致口腔內(nèi)兩大常見(jiàn)病——齲病和牙周病的始動(dòng)因子 ......
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