關(guān)鍵詞:粘性放線菌;菌毛
摘要 目的:本實(shí)驗(yàn)旨在建立一套提取和鑒定粘性放線菌菌毛的方法。方法:采用粘性放線菌變異株5519(1+ 2- )和5951(1- 2+ )分別提取Ⅰ型菌毛和Ⅱ型菌毛。首先用機(jī)械攪動(dòng)法分離菌毛與菌體,進(jìn)一步用凝膠過(guò)濾柱層析提取、純化兩種菌毛,并通過(guò)電鏡觀察和對(duì)流免疫電泳進(jìn)行初步鑒定。結(jié)果:證實(shí)分別提取到Ⅰ型菌毛和Ⅱ型菌毛。結(jié)論:兩種菌毛的提取為進(jìn)一步分析研究它們的生化特征和生物活性奠定了基礎(chǔ)。
A Study on the BiologicalProperties of Fimbriae of Actinomyces viscosus
Ⅰ . The Preparationand Determination of Fimbriae of A. viscosus
Li Wen, Liu Tianjia, Yue Songling
College of Stomatology, West China University of Medical Sciences
Zhang Chongjie, Zhao Chongrong
College of Basic Medical Sciences, West China University of Medical Sciences
Abstract Objective:To build up the methods to prepare and determine the fimbriae of A. viscosus. Methods:Type 1 and 2 fimbriae were prepared from A. viscosus 5519 and 5951 respectively. Fimbriaewere isolated from bacteria cells by mechanical shaking, obtained by collecting thesupernatant after centrifugation, and purified by ammonium sulfate precipitations and gel fitration. Results: Electron microscope showed that fimbriae existed inthe preparations. The immunological reaction between the fimbriae preparations andspecific antibodies against fimbriae attested that they were type 1 or type 2 fimbriae. Conclusion:The present study become a foundation for further study on the biological properties offimbriae of A. viscosus.
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