關(guān)鍵詞:色素上皮,眼細(xì)胞,培養(yǎng)的透明質(zhì)酸/分析流式細(xì)胞術(shù);玻璃體視網(wǎng)膜病,增生性
【摘要】 目的 觀察透明質(zhì)酸(hyaluronic acid,HA)刺激活性物(HA-stimulating activity,HASA)對(duì)培養(yǎng)的人視網(wǎng)膜色素上皮(retinal pigment epithelial,RPE)細(xì)胞的作用。 方法 將不同質(zhì)量濃度的HASA加入RPE細(xì)胞培養(yǎng)液,采用活細(xì)胞計(jì)數(shù)、四甲基偶氮唑鹽(tetrazolium,MTT)比色法及氚標(biāo)胸腺嘧啶核苷(3 H-thymidine,3 H-TdR)摻入法檢測(cè)細(xì)胞活力,用流式細(xì)胞儀(flow cytometry,FCM)分析RPE細(xì)胞周期。 結(jié)果 HASA在12.5~200.0 μg/ml范圍及 48小時(shí)內(nèi)對(duì)人RPE細(xì)胞的抑制存在劑量-效應(yīng)及時(shí)間-效應(yīng)關(guān)系。最大細(xì)胞抑制率約48.0%。FCM顯示HASA作用后G1 期細(xì)胞數(shù)較對(duì)照組增加7.2%,S期減少9.7%。 結(jié)論 HASA在一定時(shí)間和劑量范圍內(nèi)對(duì)人RPE細(xì)胞有抑制作用。
中國(guó)圖書(shū)資料分類法分類號(hào) R776 R329.2-332
Inhibition of cultured human retinal pigment epithelial cells by hyaluronic acid-stimulating activity
LIU Shaoshan,HUI Yannian,WANG Yusheng,et al.Department of Ophthalmology,Xijing Hospital,Fourth Military Medical University,Xian 710032,China
【Abstract】 Purpose To study inhibition effects of retinal pigment epithelial (RPE) cells by hyaluronic acid- stimulating activity(HASA). Methods The cultured human RPE cells added with a series of HASA was measured with cell counting,tetrazolium(MTT)colorimetric assay and tritium-labelled thymidine deoxyribose(3 H-TdR)incorporation assay.Flow cytometry(FCM)analysis was used to examine RPE cells cycles. Results HASA at concentrations of 12.5~200 μg/ml and within 48 hours inhibited RPE cells proliferation with a dose-dependant and time-dependant manners.The maximal inhibition rate of RPE cells by HASF was about 48.0%.FCM revealed that the cells in G1 phase increased 7.2% and cells in S phase decreased 9.7%,compared to controls. Conclusion HASA at a certain dose range and period can inhibit RPE cells proliferation.
【Key words】 Pigment epithelium of eye Cell ......
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