西洋參多糖提取新工藝研究
西洋參,,西洋參;多糖;提。幻,1材料與儀器,2實驗方法,4結(jié)論,參考文獻:
摘要:目的:將各種中藥處理新技術(shù)應(yīng)用于西洋參多糖的提取研究,并篩選出最佳提取工藝條件。方法:通過對回流提取法、微波提取法、超聲提取法、酶解-回流提取法4種方法進行對比,得出西洋參多糖提取的最佳方法:酶解-回流提取法,再以酶用量,料液比,酶解溫度,提取溫度等對酶解-回流提取法進行單因素試驗,初步確定各因素的最佳范圍。通過進一步的正交試驗,得出最佳工藝條件。結(jié)果:以水為溶劑,料液比1∶12,20 μl/g(相對西洋參干重)植物復(fù)合水解酶,50℃酶解1.5 h,100℃提取2次。結(jié)論:采用酶解輔助提取西洋參多糖,可以明顯提高多糖的提取率。關(guān)鍵詞:西洋參;多糖;提;酶
Study on New Technological Process for Extraction of Polysaccharide from Panax quinquefolium L.
CHEN Junhui, XIE Mingyong*, YI Xiuqin, ZHANG Zhongwei, NIE Shaoping
(Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047, China)
Abstract:Objective:Different extraction methods (reflux, microwave, supersonic wave, reflux combined enzyme) were used for the extraction of polysaccharide from Panax quinquefolium L. and the best technological parameters were selected.Methods:According to the results of the comparative experiments, it was found that reflux extraction combined enzyme was the most effective method in this case. By making the single factor and orthogonal experiments, the optimum technological parameters for reflux extraction combined enzyme were obtained.Results:The enzymatic reaction temperature of 50℃ for 1.5 h, the ration of material to solvent of 1∶12, the enzyme amount of 20 μl/g, the extraction times of 2 at 100 ℃.Conclusion:The enzyme assisted-extraction was useful in polysaccharide extraction of Panax Quinquefolium. L. ......
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