ጦ(du)IԸѪļѪܾoآzԭӰ
ߣ ǻ ꐽ ꐘI(y)
λ(̌t(y)W(xu)ԺˎW(xu)ң̌ 437100)
P(gun)I~IԸѪļzԭѪܾoآ
̌t(y)W(xu)ԺW(xu)(bo)000202 ժ Ҫ Ŀģ^ţ(Tau)(du)IѪԸѪļѪܾoآ(Ang )zԭӰ̽ӑҷʺęC(j)IһA(2K1C)IѪԸѪģ(yng)÷ⷨy(c)Ang (MAC)uᷨy(c)ļzԭ(MCC)@R^ļMY(ji)(gu)׃y(c)Ӌ(j)y(c)ļwSֱ(MFD)(du)MCCMFDcMACM(jn)оP(gun)Y(ji)2K1CIԸѪMCCMFDcMAC^g(sh)M(ShamM)@(P<0.01)MCCMFDcMAC@P(gun)(r=0.83230.83513P<0.01)ţ@2K1Cβ(dng)}տs(SBP)MFDMACMCC/ wرֵ(LVW/ BW)ļ(x)ʴļg|(zh)Y(ji)Փ2K1CļʺcļֲAngP(gun)ͨ^ļAngD(zhun)IԸѪҷʺ
, Ĵt(y)ˎ
ЈD̖(ho) R965 īI(xin)(bio)R(sh)a A
¾̖(ho) 1008-0635(2000)02-0081-03
The Effect of Taurine on Myocardial Local Angiotensin and
Collagen Concentration in Renovascular Hypertensive Rats
ZHENG Min WU Jiliang LI Lizhong et al
(epartment of Pharmacology,Xianning Medical College,Xianning 437100)
ABSTRACT Objective To study the protective effect of taurine on myocardial local angiotensin (Ang )and collagen concentration in renovascular hypertensive ratsand to research the mechanism of left ventricle hypertrophy.Methods Two-kidney 1-clip (2K1C) rats were used to reestablish the model of renovascular hypertension.The myo-cardial local Ang concentration (MAC) was measured by radioimmunoassay.The myocardial collagen concentration (MCC) was measured by biochemical method.The myocardial tissue structure was observed under the microscope,and the myocardial fiber diamension (MFD) was measured with micrometer.The linear correlation between MCC,MFD and MAC were analysed.Results Compared with sham operated rats,the MCC and MFD were significantly increased in 2K1C rats (P<0.01),both of which positively correlated with MAC(r=0.8323,0.83513,P<0.01).Taurine have an obvious suppressive effect on SBP,LVW/BW,MAC,MCC, MFD(P<0.001 for all these indices), myocardial cell hypertrophy and myocardial collagenous fiber productive reaction in 2K1C rats.Conclusion The myocardial local Ang may play roles in the myocardial hypertrophy in 2K1C rats .Taurine can produce good protective effect on left ventricle hypertrophy probably by decreasing the myocardial local Ang concentration.
, Ĵt(y)ˎ
KEY WORDS Taurine;Renovascular hypertensionMyocardial collagenAngiotensin
(Taurine,Tau)2-һ(H2N-CH2-CH2-SO3H)ǙC(j)w(ni)OSһNɰЏVW(xu)Ч(yng)l(f)F(xin)^õĿʧo(h)ļԼA(y)ļʴȶNˎ[1]併ļʺľwÙC(j)вȫ(sh)(yn)^˶IһA(2K1C)I(dng)}MխԸѪļѪܾoآ(Ang)zԭ׃ļY(ji)(gu)ĸ׃̽ӑIѪԸѪҷʺİl(f)C(j)Ƽጦ(du)ıo(h)C(j)
1 c
1.1(dng)ģ
, Ĵt(y)ˎ
xw180220g Wistar(ɱԺ(dng)ṩ)g(sh)ǰB(yng)һܣѪöIһA(2K1C)(jng)ģ[2](f)IѪԸѪģ͡3%ͱc(30mg/kg)ǻעип_xI(dng)}Ã(ni)0.20.3mmyAQAʹI(dng)}֪Mխ(du)(c)I(dng)}|g(sh)MͬyAg(sh)où5fλ/ֻǻע1A(y)Ⱦg(sh)14ÿܜy(c)Ѫ1g(sh)ĩβ(dng)}տs^g(sh)ǰ20mmHgҸ140mmHgߴ_Ѫγ
1.2 (sh)(yn)ֽM
ģɹSC(j)ֳɼg(sh)M(ShamM)ģͽM(2K1CM)M(TauM)ÿM8ֻTauMg(sh)܌Tau(50mg/kg.dُ(gu)hнƼl(f)չ˾)sˮί8ܺ̎zy(c)
, http://www.www.srpcoatings.com
1.3 (bio)ɼ̎
Qغi(dng)}Ѫ̎ѸȡKA(y)PBS(0.01mol/LpH7.4)ֹϴVɣȥK܇Y(ji)MȥѪܡķQȡҽMһKQغ0.5mol/L5ml15mins{43000rpmx20minȡҺ-30?zhn)y(c)AngȡϴļM2KһKQأy(c)ļzԭһKѸ4%Ķۼȩᾏ_Һй̶20%^ҹHQP-102ƬC(j)(C(j)늏Sa(chn))ƬNƬ-20Ҏ(gu)HEȾɫ
1.4 ^y(c)ָ(bio)
1.4.1 β(dng)}տs(SBP)
̈́(dng)y(c)x(t(y)ƴW(xu)W(xu))y(c)SBP
, Ĵt(y)ˎ
1.4.2 /wر(LVW/BW)
(bio)ɼ̎M(jn)вÿֻLVW/BWֵ
1.4.3 ļAng(MAC)
÷ⷨy(c)ˎُ(gu)ԱԇоˎfEМy(c)丱ܡ
1.4.4 ļzԭ(MCC)
Qȡx100mgļMÓ֬øBergman[3]y(c)ļuĝ֪ͬȵu(bio)(zhn)zԭ=uȡ8.2(mg/100mg)[4]
1.4.5 ļMY(ji)(gu)
, Ĵt(y)ˎ
MHEƬO(sh)lympus@R^y(c)ҽMY(ji)(gu)׃ͬr(sh)@ߜy(c)ļwSֱ(MFD)M(jn)@
1.5 y(tng)Ӌ(j)
нy(tng)Ӌ(j)(sh)(j)
sʾMg^tz(yn)
2 Y(ji)
2.1Tau(du)SBPLVW/BWMACMCCMFDӰҊ1
ɱ1Ҋ2K1CMSBPMACMCCMFDLVW/BW@ShamM(P<0.01)c2K1CM^TauίMSBPMCCMFDMACLVW/BW@(P<0.01)
, Ĵt(y)ˎ 1 Tau(du)SBPLVW/BWMACMCCMFDӰ(
sn=8)
SBP
(mmHg)
LVW/BW
(mg/g)
MAC
(ng/g tissue)
MCC
(ng/g tissue)
MFD
, Ĵt(y)ˎ (m)
Sham
11512
2.610.29
4.831.4
5.610.10
11.61.8
2K1C
17214*
3.220.28*
16.41.8*
, http://www.www.srpcoatings.com 7.940.15*
22.82.4*
Tau
12311
2.720.22
6.21.6
5.530.08
13.21.2
cShamM^*P<0.01c2K1CM^P<0.01
2.2 MCCMFDcSBPMACP(gun)ϵ
, http://www.www.srpcoatings.com
P(gun)2K1CMMCCMFDcSBPo@P(gun)MCCcMAC@P(gun)(r=0.8323P<0.01)MFDcMACҲP(gun)(r=0.83513P<0.01)
2.3 ļMW(xu)^Y(ji)
RҊ2K1CMļwS@ڠ(dng)}܇zԭM@TauMMFDcShamMo@zԭM@
3 ӑ Փ
һJ(rn)ļʺ^l(f)Kؓ(f)ɉӺm(yng)Է(yng)ͬr(sh)ļg|(zh)(du)L(zhng)ӵһN(yng)(yng)ǙC(j)wһϵл_(d)ĽY(ji)оȵAngͨ^ԷڡԷںͰ(ni)ļ(x)ʴg|(zh)ijwS(x)ֻ̼zԭϳͬcļʺ[5,6]
, Ĵt(y)ˎ
(sh)(yn)Y(ji)M(jn)һļzԭļ(x)ķʴcļֲAngˮƽP(gun)ʾļֲAng׃IԸѪҷʺ^Ҫ
Ѫҷʺ(LVH)Ɍ(do)SಡԺ(dng)ļ(x)ʴg|(zh)(x)ֳʹzԭϳӣļwSg|(zh)W(wng)j(lu)Y(ji)(gu)Ɖļ揈Ӳӣ협(yng)ԜpȱF(xin)揈ϵKM(jn)ӰտsK(do)˥ߡͬr(sh)zԭwSӿʹļM늵ĸʧʶһNܽѪ,D(zhun)ҷʺίˎʮҪţጦ(du)Ѫ˼(dng)õĽ(sh)(yn)Y(ji)ʾ@2K1CI(dng)}MխԸѪSBPLVW/BWͬr(sh)@2K1CļAngzԭļ(x)ʴzԭMõĽ߀ܸļY(ji)(gu)ܣD(zhun)ҷʺC(j)ƿcήļAngP(gun)ͨ^ηN;ʽʹ2K1CļAngpلtдM(jn)һо
, http://www.www.srpcoatings.com
īI(xin)
1ʯ|Ҧɳ.ļ(x)o(h).Ї(gu)ˎW(xu)ͨ(bo)1995;11(2)106
2Tasra K,Kamei C,Tagnli H,et al.Anti hypertensive effects of nisoldi-pine and reference drugs in certain types of hypertensive rats.Arzneim-Forsch/drug res198737(1)316
3Bergman I,Loxleg R.Two improved and simplified method for the spect-ropho to metric deter mination of hydroxyproline.Anal chem1963351961
, http://www.www.srpcoatings.com
4Mukherjee D,Sen S.Alteration of Cardial Collagen Phenotyes in hypertensive hypertrophy:role of blood pressure.J Mol Cell Cardiol199325185
5mӢ.KIѪܾoϵy(tng)cļʺ.ѪܲW(xu)M(jn)չ199617(6)336
6Sadoshima JI,Ixumo S.Molecular Characterization of angiotensin -induced hypertrophy of cardiac myocytes and hyperplasis of cardiac fibroblasts.Circ Res,199373413
ո2000-02-10
, http://www.www.srpcoatings.com
λ(̌t(y)W(xu)ԺˎW(xu)ң̌ 437100)
P(gun)I~IԸѪļzԭѪܾoآ
̌t(y)W(xu)ԺW(xu)(bo)000202 ժ Ҫ Ŀģ^ţ(Tau)(du)IѪԸѪļѪܾoآ(Ang )zԭӰ̽ӑҷʺęC(j)IһA(2K1C)IѪԸѪģ(yng)÷ⷨy(c)Ang (MAC)uᷨy(c)ļzԭ(MCC)@R^ļMY(ji)(gu)׃y(c)Ӌ(j)y(c)ļwSֱ(MFD)(du)MCCMFDcMACM(jn)оP(gun)Y(ji)2K1CIԸѪMCCMFDcMAC^g(sh)M(ShamM)@(P<0.01)MCCMFDcMAC@P(gun)(r=0.83230.83513P<0.01)ţ@2K1Cβ(dng)}տs(SBP)MFDMACMCC/ wرֵ(LVW/ BW)ļ(x)ʴļg|(zh)Y(ji)Փ2K1CļʺcļֲAngP(gun)ͨ^ļAngD(zhun)IԸѪҷʺ
, Ĵt(y)ˎ
ЈD̖(ho) R965 īI(xin)(bio)R(sh)a A
¾̖(ho) 1008-0635(2000)02-0081-03
The Effect of Taurine on Myocardial Local Angiotensin and
Collagen Concentration in Renovascular Hypertensive Rats
ZHENG Min WU Jiliang LI Lizhong et al
(epartment of Pharmacology,Xianning Medical College,Xianning 437100)
ABSTRACT Objective To study the protective effect of taurine on myocardial local angiotensin (Ang )and collagen concentration in renovascular hypertensive ratsand to research the mechanism of left ventricle hypertrophy.Methods Two-kidney 1-clip (2K1C) rats were used to reestablish the model of renovascular hypertension.The myo-cardial local Ang concentration (MAC) was measured by radioimmunoassay.The myocardial collagen concentration (MCC) was measured by biochemical method.The myocardial tissue structure was observed under the microscope,and the myocardial fiber diamension (MFD) was measured with micrometer.The linear correlation between MCC,MFD and MAC were analysed.Results Compared with sham operated rats,the MCC and MFD were significantly increased in 2K1C rats (P<0.01),both of which positively correlated with MAC(r=0.8323,0.83513,P<0.01).Taurine have an obvious suppressive effect on SBP,LVW/BW,MAC,MCC, MFD(P<0.001 for all these indices), myocardial cell hypertrophy and myocardial collagenous fiber productive reaction in 2K1C rats.Conclusion The myocardial local Ang may play roles in the myocardial hypertrophy in 2K1C rats .Taurine can produce good protective effect on left ventricle hypertrophy probably by decreasing the myocardial local Ang concentration.
, Ĵt(y)ˎ
KEY WORDS Taurine;Renovascular hypertensionMyocardial collagenAngiotensin
(Taurine,Tau)2-һ(H2N-CH2-CH2-SO3H)ǙC(j)w(ni)OSһNɰЏVW(xu)Ч(yng)l(f)F(xin)^õĿʧo(h)ļԼA(y)ļʴȶNˎ[1]併ļʺľwÙC(j)вȫ(sh)(yn)^˶IһA(2K1C)I(dng)}MխԸѪļѪܾoآ(Ang)zԭ׃ļY(ji)(gu)ĸ׃̽ӑIѪԸѪҷʺİl(f)C(j)Ƽጦ(du)ıo(h)C(j)
1 c
1.1(dng)ģ
, Ĵt(y)ˎ
xw180220g Wistar(ɱԺ(dng)ṩ)g(sh)ǰB(yng)һܣѪöIһA(2K1C)(jng)ģ[2](f)IѪԸѪģ͡3%ͱc(30mg/kg)ǻעип_xI(dng)}Ã(ni)0.20.3mmyAQAʹI(dng)}֪Mխ(du)(c)I(dng)}|g(sh)MͬyAg(sh)où5fλ/ֻǻע1A(y)Ⱦg(sh)14ÿܜy(c)Ѫ1g(sh)ĩβ(dng)}տs^g(sh)ǰ20mmHgҸ140mmHgߴ_Ѫγ
1.2 (sh)(yn)ֽM
ģɹSC(j)ֳɼg(sh)M(ShamM)ģͽM(2K1CM)M(TauM)ÿM8ֻTauMg(sh)܌Tau(50mg/kg.dُ(gu)hнƼl(f)չ˾)sˮί8ܺ̎zy(c)
, http://www.www.srpcoatings.com
1.3 (bio)ɼ̎
Qغi(dng)}Ѫ̎ѸȡKA(y)PBS(0.01mol/LpH7.4)ֹϴVɣȥK܇Y(ji)MȥѪܡķQȡҽMһKQغ0.5mol/L5ml15mins{43000rpmx20minȡҺ-30?zhn)y(c)AngȡϴļM2KһKQأy(c)ļzԭһKѸ4%Ķۼȩᾏ_Һй̶20%^ҹHQP-102ƬC(j)(C(j)늏Sa(chn))ƬNƬ-20Ҏ(gu)HEȾɫ
1.4 ^y(c)ָ(bio)
1.4.1 β(dng)}տs(SBP)
̈́(dng)y(c)x(t(y)ƴW(xu)W(xu))y(c)SBP
, Ĵt(y)ˎ
1.4.2 /wر(LVW/BW)
(bio)ɼ̎M(jn)вÿֻLVW/BWֵ
1.4.3 ļAng(MAC)
÷ⷨy(c)ˎُ(gu)ԱԇоˎfEМy(c)丱ܡ
1.4.4 ļzԭ(MCC)
Qȡx100mgļMÓ֬øBergman[3]y(c)ļuĝ֪ͬȵu(bio)(zhn)zԭ=uȡ8.2(mg/100mg)[4]
1.4.5 ļMY(ji)(gu)
, Ĵt(y)ˎ
MHEƬO(sh)lympus@R^y(c)ҽMY(ji)(gu)׃ͬr(sh)@ߜy(c)ļwSֱ(MFD)M(jn)@
1.5 y(tng)Ӌ(j)
нy(tng)Ӌ(j)(sh)(j)
2 Y(ji)
2.1Tau(du)SBPLVW/BWMACMCCMFDӰҊ1
ɱ1Ҋ2K1CMSBPMACMCCMFDLVW/BW@ShamM(P<0.01)c2K1CM^TauίMSBPMCCMFDMACLVW/BW@(P<0.01)
, Ĵt(y)ˎ 1 Tau(du)SBPLVW/BWMACMCCMFDӰ(
SBP
(mmHg)
LVW/BW
(mg/g)
MAC
(ng/g tissue)
MCC
(ng/g tissue)
MFD
, Ĵt(y)ˎ (m)
Sham
11512
2.610.29
4.831.4
5.610.10
11.61.8
2K1C
17214*
3.220.28*
16.41.8*
, http://www.www.srpcoatings.com 7.940.15*
22.82.4*
Tau
12311
2.720.22
6.21.6
5.530.08
13.21.2
cShamM^*P<0.01c2K1CM^P<0.01
2.2 MCCMFDcSBPMACP(gun)ϵ
, http://www.www.srpcoatings.com
P(gun)2K1CMMCCMFDcSBPo@P(gun)MCCcMAC@P(gun)(r=0.8323P<0.01)MFDcMACҲP(gun)(r=0.83513P<0.01)
2.3 ļMW(xu)^Y(ji)
RҊ2K1CMļwS@ڠ(dng)}܇zԭM@TauMMFDcShamMo@zԭM@
3 ӑ Փ
һJ(rn)ļʺ^l(f)Kؓ(f)ɉӺm(yng)Է(yng)ͬr(sh)ļg|(zh)(du)L(zhng)ӵһN(yng)(yng)ǙC(j)wһϵл_(d)ĽY(ji)оȵAngͨ^ԷڡԷںͰ(ni)ļ(x)ʴg|(zh)ijwS(x)ֻ̼zԭϳͬcļʺ[5,6]
, Ĵt(y)ˎ
(sh)(yn)Y(ji)M(jn)һļzԭļ(x)ķʴcļֲAngˮƽP(gun)ʾļֲAng׃IԸѪҷʺ^Ҫ
Ѫҷʺ(LVH)Ɍ(do)SಡԺ(dng)ļ(x)ʴg|(zh)(x)ֳʹzԭϳӣļwSg|(zh)W(wng)j(lu)Y(ji)(gu)Ɖļ揈Ӳӣ협(yng)ԜpȱF(xin)揈ϵKM(jn)ӰտsK(do)˥ߡͬr(sh)zԭwSӿʹļM늵ĸʧʶһNܽѪ,D(zhun)ҷʺίˎʮҪţጦ(du)Ѫ˼(dng)õĽ(sh)(yn)Y(ji)ʾ@2K1CI(dng)}MխԸѪSBPLVW/BWͬr(sh)@2K1CļAngzԭļ(x)ʴzԭMõĽ߀ܸļY(ji)(gu)ܣD(zhun)ҷʺC(j)ƿcήļAngP(gun)ͨ^ηN;ʽʹ2K1CļAngpلtдM(jn)һо
, http://www.www.srpcoatings.com
īI(xin)
1ʯ|Ҧɳ.ļ(x)o(h).Ї(gu)ˎW(xu)ͨ(bo)1995;11(2)106
2Tasra K,Kamei C,Tagnli H,et al.Anti hypertensive effects of nisoldi-pine and reference drugs in certain types of hypertensive rats.Arzneim-Forsch/drug res198737(1)316
3Bergman I,Loxleg R.Two improved and simplified method for the spect-ropho to metric deter mination of hydroxyproline.Anal chem1963351961
, http://www.www.srpcoatings.com
4Mukherjee D,Sen S.Alteration of Cardial Collagen Phenotyes in hypertensive hypertrophy:role of blood pressure.J Mol Cell Cardiol199325185
5mӢ.KIѪܾoϵy(tng)cļʺ.ѪܲW(xu)M(jn)չ199617(6)336
6Sadoshima JI,Ixumo S.Molecular Characterization of angiotensin -induced hypertrophy of cardiac myocytes and hyperplasis of cardiac fibroblasts.Circ Res,199373413
ո2000-02-10
, http://www.www.srpcoatings.com