5—ALA—納米金光動力治療小鼠光老化的初步研究(1)
[摘要]目的：初步探討5-ALA-納米金光動力治療小鼠皮膚光老化的作用。方法：48只雄性健康ICR小鼠分為正常對照組(8只)、光老化組(40只)，正常組不予特殊處理，光老化組照射紫外線，肉眼觀察和HE染色驗證光老化模型。40只光老化組小鼠分為B組光老化對照組，C組紅光組、D組納米金組、E組5-ALA組、F組5-ALA-納米金組，C、D、E、F組小鼠背部分別涂抹不同溶液0.5ml(分別為生理鹽水、納米金溶液、5-ALA溶液和5-ALA-納米金組溶液)避光封包3h，紅光照射10min，2周治療1次，共治療2次，A組為正常組對照組不予任何處理。觀察各組小鼠皮膚表現(xiàn)和組織病理改變。結果：與A組比較，紫外線照射后，B組小鼠皮膚皮膚增厚，出現(xiàn)皮屑、深皺紋、缺乏彈性等光老化特征；組織切片顯示，B組小鼠表皮厚度[(168.235±13.665)μm]較A組[表皮(87.165±2.627)μm]增厚(P<0.05)，而膠原纖維面積密度[(7.390±2.5)%]均較A組[(40.114±3.0)%]明顯減少(P<0.05)。與B組相比，C、D、E和F組小鼠的表皮變薄[分別為(154.458±2.942)μm，(114.958±2.229)μm，(50.623±4.203)μm和(31.694±1.970)μm，P<0.05]，膠原纖維面積密度增加[分別為(15.840±3.0)%，(27.320±2.5)%，(60.812±2.0)%和(70.024±3.0)%，P<0.05]，其中以F組效果最顯著。結論：5-ALA結合體能提高光動力療法治療小鼠光老化的療效，同時納米金的光熱作用可能用于治療光老化。
, 百拇醫(yī)藥
[關鍵詞]皮膚；光老化；光動力；納米金；5-氨基乙酰丙酸；HE染色；膠原纖維；小鼠
[中圖分類號]R751 [文獻標志碼]A [文章編號]1008-6455(2018)11-0137-04
Abstract： Objective To primarily discuss the effects of using 5-ALA- nanogold for the treatment of photoaged mice irradiated with 640nm red light. Methods 48 ICR male mice are randomly divided into normal control group (8 mice) and photoaging group (40 mice) with ultraviolet irradiation，then we verify the success of photoaging mice models utilizing HE staining and gross inspection.Second A total of 40 photoaging mice are equally devided in five groups： photoaging control group(group B) receiving no treatment， red laser group (group C)，nanogolds group (group D)，5-ALA group (group E)and 5-ALA-nanogold group(group F).The dorsal skin of group C，D，E and F’ mice are applied 0.5ml different kinds of solutions (respectively normal saline， nanogald， 5-ALA and 5-ALA-nanogold)， then receiving red light irradiation for 10min after covering with plastic mulch for 3 hours， and received a second treatment after two weeks. The normal control group without treatment is named as group A. The dorsal skin of each group mice are analysed by clinical manifestations and histopathologic changes. Results Compared with group A， the mice skin of group B shows the photoaging charactristics such as thickened and hardened skin， loss of resilience， deep winkles and dandruffs affter irradiation from UV. HE staining show the epidermis thickness of group B[(168.235±13.665)μm] is significantly thickener than group A[(87.165±2.627)μm] (P<0.05)， and the collagen fiber area density of group B [(7.390±2.5)%] is significantly reduction than group A [(40.114±3.0)%] (P<0.05). The epidermis thickness of group C， D， E， F are respectively thinner than group B[respectivly (154.458±2.942)μm，(114.958±2.229)μm，(50.623±4.203)μm and (31.694±1.970)μm，P<0.05] and the collagen fiber area density of group C， D， E， F are significantly on the increase [respectively (15.840±3.0)%， (27.320±2.5)%， (60.812±2.0)% and (70.024±3.0)%， P<0.05] than group B， of which the most significant effect is group F. Conclusion 5-ALA and nanogold can cooperativly effect photodynamic therapy for photoaging mice skin and the photothermal effect of nanogold may be able to treat skin photoaging., 百拇醫(yī)藥(葛芹 劉亞樂 李張軍)
, 百拇醫(yī)藥
[關鍵詞]皮膚；光老化；光動力；納米金；5-氨基乙酰丙酸；HE染色；膠原纖維；小鼠
[中圖分類號]R751 [文獻標志碼]A [文章編號]1008-6455(2018)11-0137-04
Abstract： Objective To primarily discuss the effects of using 5-ALA- nanogold for the treatment of photoaged mice irradiated with 640nm red light. Methods 48 ICR male mice are randomly divided into normal control group (8 mice) and photoaging group (40 mice) with ultraviolet irradiation，then we verify the success of photoaging mice models utilizing HE staining and gross inspection.Second A total of 40 photoaging mice are equally devided in five groups： photoaging control group(group B) receiving no treatment， red laser group (group C)，nanogolds group (group D)，5-ALA group (group E)and 5-ALA-nanogold group(group F).The dorsal skin of group C，D，E and F’ mice are applied 0.5ml different kinds of solutions (respectively normal saline， nanogald， 5-ALA and 5-ALA-nanogold)， then receiving red light irradiation for 10min after covering with plastic mulch for 3 hours， and received a second treatment after two weeks. The normal control group without treatment is named as group A. The dorsal skin of each group mice are analysed by clinical manifestations and histopathologic changes. Results Compared with group A， the mice skin of group B shows the photoaging charactristics such as thickened and hardened skin， loss of resilience， deep winkles and dandruffs affter irradiation from UV. HE staining show the epidermis thickness of group B[(168.235±13.665)μm] is significantly thickener than group A[(87.165±2.627)μm] (P<0.05)， and the collagen fiber area density of group B [(7.390±2.5)%] is significantly reduction than group A [(40.114±3.0)%] (P<0.05). The epidermis thickness of group C， D， E， F are respectively thinner than group B[respectivly (154.458±2.942)μm，(114.958±2.229)μm，(50.623±4.203)μm and (31.694±1.970)μm，P<0.05] and the collagen fiber area density of group C， D， E， F are significantly on the increase [respectively (15.840±3.0)%， (27.320±2.5)%， (60.812±2.0)% and (70.024±3.0)%， P<0.05] than group B， of which the most significant effect is group F. Conclusion 5-ALA and nanogold can cooperativly effect photodynamic therapy for photoaging mice skin and the photothermal effect of nanogold may be able to treat skin photoaging., 百拇醫(yī)藥(葛芹 劉亞樂 李張軍)
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